Mass spectrometry (MS) is an analytical technique that allows for identification of biomolecules and the determination of their elemental composition. Combination of Electrospray MS and Hydrogen-deuterium exchange tracking also allows for a detailed structural analysis of macromolecules, such as peptides and proteins.
Available instruments include Waters Synapt G1 and Synapt G2 Mass Spectrometers.
Nuclear Magnetic Resonance Spectroscopy
Nuclear Magnetic Resonance (NMR) spectroscopy is a technique which exploits the magnetic properties of certain atomic nuclei to uncover information on chemical structures. Modern multi-dimensional NMR methods that utilize isotopically enriched biomolecules can be used to determine the three-dimensional structures of such biomolecules in complexes.
Available instruments include Varian Inova 600 (access kindly provided by Dr. Michael Organ) and AV III 700 (access kindly provided by Dr. Howard Hunter) NMR spectrometers.
X-ray Crystallography is a method that employs X-ray diffraction patterns to determine the arrangement of atoms within a crystal. X-ray crystallography can produce three-dimensional maps of the atomic positions and the types of chemical bonds between them for macromolecules and their complexes.
Available instrumentation includes a Rigaku MicroMax 007HF with Saturn 944+ CCD detector (access kindly provided by Drs. Gerald Audette & Vivian Saridakis).
Capillary Electrophoresis (CE) is a versatile platform that allows for a high-efficiency separation of biomolecules for both preparative and analytical purposes. CE is an effective method for selection and screening on interacting biomolecular pairs, characterization of kinetics of molecular interactions, sequencing of biological polymers and purity analysis of biological and pharmaceutical samples.
Available instruments include Beckman Coulter (P/ACE MDQ, ProteomeLab PA800, and CESI 8000) as well as Sciex (P/ACE MDQ plus) capillary electrophoresis systems.
Flow cytometry is a technique that allows for a multiparametric characterization of populations of biological microparticles, such as cells, in a high-throughput fashion. With the use of various biological dyes and biomarker-specific ligands, flow cytometry can achieve simultaneous analysis of the physical characteristics and biological states of large cell populations.
Available instruments include BD FACS Canto II Flow Cytometer.
Chemical cytometry is a technique that allows for qualitative and quantitative analysis of the chemical contents of single cells. Analysis on a single-cell level is required when spatially-related cell sub-populations are highly heterogeneous in respect to some of their properties. Cell cytometry enables such high-sensitivity characterizations of heterogeneous cell populations, which are often present in such biological processes as tissue regeneration, disease and cancer.
Available instruments include custom-made single- and multi-channel chemical cytometry systems.
Research on biomolecules often requires their availability in a purified form. Members of CRBI have access to a wide range of tools and instruments dedicated to biomolecular purification, including Pharmacia AKTA FPLC system and Beckman Coulter SystemGold HPLC system.
Cutting-edge research often requires fabrication of custom made systems and apparatuses. Members of CRBI have access to a Roland MDX-540 Pro-II Milling Machine and a VersaLaser laser cutting system, which are used for fabrication of systems ranging from microfluidic devices to custom-made fluorescence detectors.
- Olympus FluoView Confocal Microscopy system
- Various Light and Fluorescence Microscopy systems, including a system with a Sutter Instrument XenoWorks Micromanipulator & Microinjector System.
- MicroCal VP-Isothermal Titration Calorimeter
- Bio-Rad iCycler Real Time PCR system
This is by no means a full inventory of the available tools and instruments. In addition to the list of instruments above, the members of CRBI posses a wide variety of more common tools and facilities directed towards biological research.