CRBI is hosting a series of seminars that introduce the research topics currently pursued in the laboratories of the members of the centre. In the second round of seminars, the postdoctoral fellows and graduate students will introduce narrower research topics in more detail.

David Wegman, from Dr. Sergey Krylov's research group, will present his talk, titled

"Direct Quantitative Analysis of Multiple micro-RNA (DQAMmiR)"

Abstract:

MicroRNAs (miRNAs) are short RNA molecules (18-25 nucleotides) that play an important role in the regulation of cellular processes and their abnormal expression is associated with several diseases, including cancer. We have developed the first direct quantitative analysis of multiple miRNAs (DQAMmiR). In a single experiment, DQAMmiR can accurately determine the concentration of multiple miRNA species directly, without any modifications to the miRNA. DQAMmiR is a hybridization assay that uses complementary, fluorescently-labeled DNA probes that are mixed with a sample containing the target miRNA species. Capillary electrophoresis with laser-induced fluorescence (CE-LIF) is used to separate and detect the miRNA-DNA probe hybrids. For this proof-of-principle work, we developed DQAMmiR for 3 miRNAs (mir21, 125b, 145) which are known to be deregulated in breast cancer. DQAMmiR offers the opportunity for simple and fast quantitative fingerprinting of miRNAs in basic research and clinical applications.

 

Please join us,

Refreshments will be served.