Centre for Research on Biomolecular Interactions presents Prof. Han Bin Oh, Department of Chemistry, Sogang University, Seoul, South Korea

Talk Title: "Free radical initiated peptide sequencing (FRIPS) mass spectrometry and the additional efforts towards building 'smart' mass spectrometry laboratory"

Abstract:

As another nice version of radical-based peptide tandem mass spectrometry, we have developed the so-called TEMPO-assisted FRIPS mass spectrometry method: TEMPO, (2,2,6,6-Tetramethylpiperidin-1-yl)oxyl. We synthesized o-TEMPO-Bz-CO-NHS and conjugated to this reagent to the N-terminus of peptides of interest. Taking advantage of the enormous thermodynamic stability of TEMPO radical, we could direct the applied collisional energy exclusively into the C-O bond homolytic cleavage, i.e., forming •Bz-CO-peptide. The generated peptide radical species have been shown to be very effective in peptide sequencing. In the seminar, our additional efforts to make our TEMPO-assisted FRIPS mass spectrometry as a practical –omics tool will be presented. In addition, we have pursued the technical development of combining the EWOD (electrowetting-on-dielectrics) with mass spectrometry. The recently acquired results of EWOD plus mass spectrometry will be also presented. Last, we have turned our attention to the use of artificial neural network computations in improving the current LC-MS/MS methods. Some fruits of this direction will also be presented.

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Refreshments will be served.